BIO218 PRACTICE QUESTIONS

In the estmation of phosphate using molybdic acid, the absorbance is read at 700nm

The blue coloration given when DNA is treated with diphenylamine under acid conditionsis due to 2- deoxypentoses In the isolation of RNA from yeast cells, phenol causes the denaturing of protein Folin-Wu method is used for estimation of glucose in a blood Cellulose replaces Silica gel used in Thin Layer Chromatography In thin layer chromatography, a small amount of sample is applied (spotted) near the bottom of the plate

and the plate is placed in the mobile phase In the isolation of RNA from yeast cells, phenol causes the denaturing of protein Cellulose can be used to replace Silica gel used in Thin Layer Chromatography Folin-Wu method is used for estimation of glucose in a blood In the Dam method, bromine is used in preference to iodine The iodine number of a lipid or fat sample is defined as the number of grams of iodine absorbed by 100 grams of lipid Folin-Wu method is used for estimation of glucose in a blood Urine contains traces of phenol which can interfere with sensitive colormetric methods In the Dam method, bromine is used in preference to iodine The general test of carbohydrates is is called Molisch test Reaction with Barfoed’s Reagent gives a red-brown colour Fehling's solution is prepared by dissolving 6.93g of coper sulphate in 100 ml of distilled water Addition of drops of dilute iodine solution to starch gives a colour which on heating disappears Urine contains traces of phenol which can interfere with sensitive colormetric methods The hydrolysis of fat by alkali is called saponification The number of grams of iodine absorbed by 100 grams of lipid is called Iodine value Complete hydrolysis of starch is proved by positive Benedict’s test

The denatured protein during RNA isolation from yeast is removed by centrifugation Further purification during the isolation of RNA from yeast can be made by treating the preparation with amylase The genetic material in viruses is called RNA The symbol "M" stands for the concentration of solutions in Molarity The symbol "N" stands for the concentration of solutions in Normality The normal blood glucose range is 4-8 mmol/L Molisch test is the general test for carbohydrates Failure of pancreas to produce sufficient insulin is a condition found in Diabetics

Reaction with Barfoed’s Reagent gives a red brown colour Further purification during the isolation of RNA from yeast can be made by treating the preparation with amylase Failure of pancreas to produce sufficient insulin is a condition found in Diabetics